By P.C. Emson, M. Rossor and M. Tohyama (Eds.)

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B) Incubation with PAP complex in PBS-NGS for 3 h at room temperature. (c) Wash in PBS for 30 min at 4°C. 6) for 30 rnin at 4°C. (b) Incubation with DAB solution (50 mg in 100 ml of Tris-HC1 buffer). 01 YO)for 3-20 rnin at room temperature. (d) Wash in Tris-HC1 buffer. 1 M phosphate buffer at room temperature. (f) Wash in phosphate buffer. Step 6 (a) Block staining by 1% uranyl acetate in 70% ethanol. (b) Dehydration with series of ethanol and propylene oxide. (c) Immersion in the propylene oxide-Epon (1:l) for 1-2 h at room temperature.

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1962) Buffered formaldehyde as a killing agent and primary fixative for electron-microscopy. Anat. , 142 342. Pearse, A. G. E. and Polak, J. M. (1975) Bifunctional reagents as vapour- and liquid-phase fixatives for immunohistochemistry. Histochem. , I: 179-186. , Weaver, C. and Keefer, D. A. (1975) Improved immunoglobulin enzyme bridge method for light-microscopic demonstration of hormone-containing cells of rat adenohypophysis. Histochemistry, 4 6 926. Pickel, V. , Joh, T. H. and Reis, D. J. (1975) Ultrastructural localization of tyrosine hydroxylase in noradrenergic neurons of brain.

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