By Ali Masoudi-Nejad, Zahra Narimani, Nazanin Hosseinkhan

The objective of this booklet is to introduce the organic and technical points of subsequent iteration sequencing equipment, in addition to algorithms to collect those sequences into complete genomes. The publication is equipped into components; half 1 introduces NGS tools and half 2 stories meeting algorithms and offers an outstanding perception to those equipment for readers new to the sector. amassing details, approximately sequencing and meeting equipment jointly, is helping either biologists and machine scientists to get a transparent proposal in regards to the box. Chapters will contain information regarding new sequencing applied sciences equivalent to ChIp-seq, ChIp-chip, and De Novo series meeting. ​

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5. Ansorge, W. J. (2009). Next-generation DNA sequencing techniques. New Biotechnology, 25(4), 195–203. 6. , et al. (2005). Accurate multiplex polony sequencing of an evolved bacterial genome. Science, 309(5741), 1728–1732. 7. Mardis, E. R. (2008). Next-generation DNA sequencing methods. Annual Review of Genomics and Human Genetics, 9, 387–402. 8. , & Ji, H. (2008). Next-generation DNA sequencing. Nature Biotechnology, 26(10), 1135–1145. 9. Berglund, E. , & Syvänen, A. C. (2011). Next-generation sequencing technologies and applications for human genetic history and forensics.

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Microbiology: Metagenomics. Nature, 455(7212), 481–483. 28. , et al. (1998). Consed: A graphical tool for sequence finishing. Genome research, 8(3), 195–202. 29. Kent, W. , Sugnet, C. , Furey, T. , Roskin, K. , Pringle, T. , Zahler, A. , Haussler, D. (2002). Genome Research, 12(6), 996–1006. 30. , et al. (2009). The sequence alignment/map format and SAMtools. Bioinformatics, 25(16), 2078–2079. 31. , et al. (2008). SOAP: Short oligonucleotide alignment program. Bioinformatics, 24(5), 713–714. 32.

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