By Albanese, A.A.

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Furthermore, the N 15 excreted in the urine and feces allows assessment to be made of the amount of tracer retained under different conditions. 5 gm of gelatin per kg of body weight admixed with I 131 -labeled albumin. Chinn and others (155) reported similar results. Nakayama et al. (158), on the other hand, showed that maximum concentrations of radioactivity in the peripheral blood were delayed to between 6 and 7 40 ANTHONY A. ALBANESE AND LOUISE A. ORTO hours in normal subjects receiving test doses of S35- and P 32 -labeled proteins along with a meal consisting of 86 gm of carbohydrate, 30 gm of fat, and 26 gm of protein.

10 · x · ». £ -10 -20 L . · I x I -30 -40 X x x xx I * x * * X X -50 N Balance in steroid period — N Balance in control period (B3 — B0) FIG. 7. Relationship of SPAI to nitrogen balance changes induced by a protein anabolic steroid (oxandrolone) and a protein catabolic steroid (triamcinolone). Re­ sults with oxandrolone are marked with solid dots, and those with triamcinolone with the symbol x. appears to be rectilinear. However, the lines become curvilinear as they are extended into the area of positive balance.

The fact that these rates may be appreciably different has been used by Bocci (163) to determine the extent of de­ naturing of I 131 -labeled gamma globulins. Work with radioactive tracers usually involves the measurement of activity of samples of whole blood and no attempt is made to separate and identify specifically labeled amino acids. Provided that one can be sure that no free iodide is present in the sample, the method is probably sufficiently accurate for work with I 131 -labeled proteins.

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