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Extra info for Biomembranes Part B
During pump-down the stage temperature is raised to - 100 ° (or - 110 ° for no etch) and the knife is cooled to - 196 °. , after 15-20 minutes) several "cuts" are made. After the last "cut" the knife is left above the specimen during etching. After etching the knife is moved and the Pt-C electrode is fired, then the C-C electrode. The knife is returned to a position above the sampies, the jar is brought to atmospheric pressure and raised. The knife is moved aside, the stage cap is quickly unscrewed, and the disk is grasped with forceps, warmed, and slid into water, bleach, or acid in a small petri dish.
The technique of optical diffraction will indicate the extent to which this random component contributes to the image, while the technique of optical filtering can be used with regularly repeating structures both to eliminate much of the "noise" and to obtain the image from one side of an object free of that from the other. ,3s Quantitative Studies. These range from, for example, estimating the proportion of contaminating membrane fragments in a preparation of mitochondria, through counts of the characteristic structures formed when complement is bound to membranes, to determination of the size of macromolecular structures and the number of subunits that they contain.
Plant Physiol. 20, 209 (1969). ~V. Luzzati, in "Biological Membranes" (D. ), p. 71. Academic Press, New York, 1968. 5D. Deamer, R. Leonard, A. Tardieu, and D. Branton, Biochim. Biophys. Acta 219, 47 (1970). 46 CHARACTERIZATION OF MEMBRANES AND COMPONENTS  3. The fracture face should be shadowed and replicated immediately after the final fracture. Do not include an etching period unless further information may be obtained by sublimation of ice from the fracture face. Cleaning Replicas Replicas of pure lipid systems may be quickly cleaned in organic solvents--for instance, chloroform-methanol.