By Willis A. Wood, Scott T. Kellogg
This quantity covers cellulose and hemicellulose and comprises confirmed and reproducible tools for examine on the topic of the conversion of carbohydrate polymers to usable monomeric devices. Sections at the coaching of biomass fabrics and of substrates are integrated, as are sections on analytical tools and at the purification and assay of enzymes
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Extra info for Biomass Part A: Cellulose and Hemicellulose
M. Wood, Biochem. J. 121, 353 (1971). 24 CELLULOSE  Preparation of Regenerated Cellulose Cotton fiber (1 g) is suspended in 100 ml 70% (w/v) H3PO4 and the mixture heated with stirring at 60 ° for 4 hr. The clear solution is poured, with stirring, into 1 liter of a mixture of ice and water. The precipitated cellulose is allowed to settle and is then washed by decantation with several liters of water until neutral. 05 M with the bacteriocide: the suspension is kept in this state at 4 °. No attempt should be made to dry the cellulose as it has been found to rehydrate only very slowly and to be much less susceptible to cellulase action than never-dried material.
4° Preparation of the solvent is relatively simple. One of the best descriptions is the preparation of Segal and Tinlpa. 41 Solvent preparation is described below. Cadmium oxide should be oven dried at 110° and stored in a desiccator. Sodium hydroxide pellets are added to ethylenediamine and allowed to stand at least 24 hr. The ethylenediamine should then be distilled over sodium hydroxide pellets just prior to use. (The use of freshly distilled 36 M. R. Ladisch, C. M. Ladisch, and G. T. Tsao, Science 201, 743 (1978).
36 Comparison of the resulting spectra with published data should show similar spectra. Otherwise, a chemically impure cellodextrin should be suspected. A comparison of the infrared spectra of cellodextrins prepared by the HC1 (Fig. 2a) and by H2SO4 hydrolysis of cellulose (Fig. 2b) demonstrates an unidentified peak at 1210-1260 cm -I in the latter spectrum. Otherwise, the rest of the peaks compare well with published data. 36 13C NMR spectra of cellodextrins prepared in our laboratory are given in Figs.