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W. Boyer, Proc. Natl. Acad. Sci. A. 72, 3310 (1975). i C. J. , O. Hagenbuchle, and P. H. von Hippel, J. Biol. Chem. 255, 11534 (1980). J T. I. Tikchonenko, E. V. Karamov, B. A. Zavizion, and B. S. Naroditsky, Gene 4, 195 (1978). k M. Hsu, and P. Berg, Biochemistry 17, 131 (1978). H. Belle Isle, unpublished results, 1981. 32 ENZYMES IN RECOMBINANT D N A [1] should be performed under the recommended standard assay conditions especially in regard to pH, ionic strength, and divalent cation concentration.

J. 177, 49 (1979). K. Heininger, W. Horz, and H. G. Zachau, Gene 1, 291 (1977). h B. Polisky, P. Greene, D. E. Garfin, B. J. McCarthy, H. M. Goodman, and H. W. Boyer, Proc. Natl. Acad. Sci. A. 72, 3310 (1975). i C. J. , O. Hagenbuchle, and P. H. von Hippel, J. Biol. Chem. 255, 11534 (1980). J T. I. Tikchonenko, E. V. Karamov, B. A. Zavizion, and B. S. Naroditsky, Gene 4, 195 (1978). k M. Hsu, and P. Berg, Biochemistry 17, 131 (1978). H. Belle Isle, unpublished results, 1981. 32 ENZYMES IN RECOMBINANT D N A [1] should be performed under the recommended standard assay conditions especially in regard to pH, ionic strength, and divalent cation concentration.

The sensitivity of restriction endonuclease cleavage to methylation (Table IV) can be used advantageously to deduce the patterns and the extent of methylation in DNA. For example, the differential reactivity of the isoschizomers MspI and HpalI to mCG was used to identify gross tissue specific differences in methylation patterns and, more important, to identify the methylation status of cleavage sites within a specific gene or genetic region. 62 6o M. Ehrlich and R. -H. Wang, Science 212, 1350 (1981).

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