By Jun Hirabayashi

Lectins: equipment and Protocols summarizes vintage lectin applied sciences and complex ideas with excessive throughputs and sensitivities. Chapters contain equipment and methods for serial lectin-affinity chromatography process, lectin-probed western blot and histochemical analyses, quantitative interplay analyses in accordance with equilibrium dialysis, isothermal calorimetry, floor plasmon resonance, ideas for elucidating services of endogenous animal lectins, complicated equipment on “engineer” novel lectins by means of evolutionary techniques, and particular gear. Written within the hugely winning Methods in Molecular Biology series layout, chapters contain introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, effortlessly reproducible laboratory protocols, and key tips about troubleshooting and heading off recognized pitfalls.

Authoritative and useful, Lectins: equipment and Protocols seeks to assist not just lectin experts but additionally non-experts together with either younger scientists.

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Fungal Lectins 29 The fucose-specific plant and fungal lectins summarized in this chapter may prove to be useful for the detection of AFP-L3 and other new biomarkers, and for determining the physiological functions of oligosaccharides. 4 Classification of Fungal Lectins The amino acid sequences of several hundreds of lectins have now been established, and many of their three-dimensional structures have also been elucidated. Recently, a classification system was proposed, which subdivided these lectins into 48 families of structurally and evolutionarily related proteins (see Chapter 46 by Z.

However, how many of these methods are scientifically supported? Lectins, enzyme inhibitors of animal digestive enzymes, tannins, and phytates have been called “antinutrients” because they decrease the absorption of nutrients. These antinutrients are classified as self-defense proteins in plants, and most legume lectins are considered to cause intestinal inflammation in animals when consumed without heat treatment. ), Lectins: Methods and Protocols, Methods in Molecular Biology, vol. 1007/978-1-4939-1292-6_3, © Springer Science+Business Media New York 2014 39 40 Haruko Ogawa and Kimie Date E-PHA (or PHA-E4) and L-PHA (or PHA-L4) are the most commonly used in structural carbohydrate chemistry owing to their unique specificities for multiantennary complex-type N-glycans [1].

Chachadi VB, Inamdar SR, Yu LG et al (2011) Exquisite binding specificity of Sclerotium rolfsii lectin toward TF-related O-linked mucin-type glycans. Glycoconj J 28:49–56 38 Yuka Kobayashi and Hirokazu Kawagishi 135. Inbar J, Chet I (1994) A newly isolated lectin from the plant pathogenic fungus Sclerotium rolfsii: purification, characterization and role in mycoparasitism. Microbiology 140(Pt 3): 651–657 136. Leonidas DD, Swamy BM, Hatzopoulos GN et al (2007) Structural basis for the carbohydrate recognition of the Sclerotium rolfsii lectin.

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