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History of, 290±291 motility of, 305±308 msDNA from, 296±297 mutagenesis of, 303±305 sporulation of, 312±317 survival in nature of, 291 transduction in, 297±301 transposons of, 301±303 Myxobacteria, generalized transduction in, 562 Myxococcus, Escherichia coli versus, 181 Myxococcus xanthus, 291±317 carotenoids in, 307±308 constructing deletion mutants of, 303±305 cotransduction in genetic mapping of, 299, 300 fruiting bodies of, 291±294, 294±295, 312±317 genome of, 295±297 gliding by, 308±312 gliding genes in, 310±312 gliding motors in, 310 heat-resistant spores of, 316±317 motility of, 305±308, 308±312 msDNA from, 296±297 nonmotile mutants of, 310±312 overriding quorum sensing in, 269 regulatory units in, 306±307 s factors of, 305±306 sporogenesis in, 293±294 sporulation of, 312±317, 357 strains of, 295 survival in nature of, 291 transduction into, 297±301 transposons in, 295±296, 299, 301±303 vegetative growth of, 294 Myxococcus xanthus A plasmid, regulatory elements in, 306 Myxococcus xanthus strain DK101, in electroporation, 300, 300 n Myxococcus xanthus strain DK6204, constructing, 304 Myxophage Mx4 in Myxococcus xanthus cotransduction, 299 Myxococcus xanthus motility and, 310 in Myxococcus xanthus transduction, 297, 298 Myxophage Mx8 in Myxococcus xanthus cotransduction, 299 Myxococcus xanthus motility and, 310, 312 in Myxococcus xanthus transduction, 297, 298±299 Myxothiazol, from Stigmatella aurantiaca, 294 Myxovirescin (antibiotic TA), from myxobacteria, 294 N block, in sensor protein transmitter domain, 351 N gene, of bacteriophage l, 132 N protein, of bacteriophage l, 67, 132±133 N15 labeling, in proving semiconservative DNA replication, 4±5 N4mC (N4-methylcytosine) methylation, 197±198, 199 target-recognizing domains for, 209 N6mA (N6-methyladenine) methylation, 197±198, 199, 200 target-recognizing domains for, 209 NAD‡ -dependent alcohol dehydrogenase, in Myxococcus xanthus sporulation, 315 Nae I endonuclease, operation of, 202±203 Naegleria gruberi, plasmids in, 540 Nannocystis exedens, survival in nature of, 291 NarL protein, of Escherichia coli, 372 Nathans, Daniel discovery of restriction enzymes by, 178, 179 molecular cloning and, 243 Natural competence, 450±451 NBU1 transposon, 390±392, 415±416 NBU2 transposon, 415±416 NBUs (nonreplicating Bacteroides units), as mobilizable transposons, 415±417 ndd (nuclear disruption defective) gene, in bacteriophage T4 infection, 108 Negative control, plasmids and, 510, 544 Neisseria binding in, 441 competence in, 433, 438, 439 DNA uptake in, 443 phase variation in, 77 type III restriction-modification systems in, 194 Neisseria gonorrhoeae binding in, 442 DNA uptake in, 445 phase variation in, 76 Neisseria meningitidis, restriction-modification system of, 184±185 Nematodes, reduced Pseudomonas aeruginosa virulence in, 265 Neurospora, methyltransferases in, 200 New England BioLabs, 245 N-glycosylic bonds, BER hydrolysis of, 34±35 Nicotiana glauca, Agrobacterium tumefaciens tumorigenesis in, 327 Nicotiana tabacum, inoculated with mutant Agrobacterium tumefaciens strains, 324 9 mers, in replication initiation, 6±7 Nitrogen, in proving semiconservative DNA replication, 4±5 INDEX Nitrogen fixation, 181 N-methyl-N0 -nitro-N-nitrosoguanidine (MNNG), adaptive repair of DNA damage from, 42 Noncoding lesions.

INDEX Excisive transposition, 387, 388 Exconjugants, after conjugation, 465 Exocyclic amino groups, of DNA bases, 30 Exons of bacteriophage T4, 117±118 transposons and, 390±392 Exonucleases in base excision repair, 35 in nucleotide excision repair, 32 in recombination, 231 Exopolysaccharide (EPS), in Myxococcus xanthus social motility, 308, 310 Exotoxin A, in Pseudomonas aeruginosa virulence, 265 ExpREch protein, of Erwinia chrysanthemi, 374 Expressed genes. See also Gene expression repair bias toward, 33 in transformation, 431 Expressed sequence tags (ESTs), in cloning, 252 Expression vectors, table of, 584 Extracytoplasmic functions (ECF), of s factors, 63, 64, 305 F block, in sensor protein transmitter domain, 351 F conjugative plasmids, 147.

See also Genomes for antibiotic resistance, 412 n of bacteriophage w29, 122 of bacteriophage T4, 92 in bacteriophage T4 infections, 107±113 within bacteriophage T4 introns, 117±118 of clonal organisms, 181±182 complementation among, 99±100 for DNA polymerase III, 13 in Escherichia coli replication and repair, 11 in F factor replicator, 249 of filamentous bacteriophage, 152±154 homing endonuclease, 205±206 identifying with broad host cloning, 595 introns in bacteriophage T4, 115±119 of isometric bacteriophage, 149±152 in linkage, 454±454 mobility of restriction-modification system, 210 of myxobacteria, 291 nonhomologous recombination of, 139±140 recombination of, 227±240 repair biases toward, 33 screening via homologous, 250 for s factors, 50 stress shock, 281±285 in transcriptional regulation, 56±57 in translation, 212 in transposons, 389, 390±392 Genetic engineering Agrobacterium tumefaciens in, 323 molecular cloning in, 244 natural, 325±326 progress in, 340 through Agrobacterium tumefaciens, 336±340 Genetic load, 27 Genetic markers with bacteriophage, 96±97 in cloning vectors, 246 in conjugational mapping, 497±499 cotransduction of, 570±572 in gene mapping transformation, 459±460 Genetic mosaics, lambdoid phages as, 139 Genetically modified organisms (GMOs) Agrobacterium tumefaciens and, 340 from molecular cloning, 244 Genomes.

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