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Additional info for Biomembranes Part G: Bioenergetics: Biogenesis of Mitochondria, Organization, and Transport
L° Maximum incorporation rates were obtained when glutamate and the specific inhibitor of the adenine translocase, atractyloside, were added in the absence of exogenous nucleotides. The rate of amino acid incorporation under these conditions was ten times that obtained when the energy source was the external ATP-regenerating system and five times that when it was glutamate and ADP. These results suggest that, in tightly coupled mitochondria, the combination of atractyloside and substrate may be the best way to achieve a maximum internal ATP concentration.
X. Balcavage, Vol. 10, 135. 26 G. Schatz, Vol. 10, 197. 27 R. B. Needleman and A. Tzagoloff, Anal. Biochem. 64, 545 (1975). 2s H. T. Hutchison and L. H. Hartwell, J. Bacteriol. 94, 1976 (1967). 29 W. Birchmeier, C. Kohler, and G. Schatz, Proc. Natl. Acad. Sci. A. 73, 4334 (1976). 30 G. Coruzzi, M. K. Trembath, and A. Tzagoloff, this volume . al M. Douglas and R. A. Butow, Proc. Natl. Acad. Sci. A. 73, 1083 (1976).  BIOGENESIS OF YEAST MITOCHONDRIA 45 and Butow 31 and Schatz and Saltzgaber.
Chem. 251, 1997 (1976). ~1 p. p. " Masson, Paris, 1953. lz T. Sugimura, K. Okabe, M. Nagao, and N. Gunge, Biochim. Biophys. Acta 115, 267 (1966). 13 E. G. Golub, P. Trocha, P. K. Liu, and D. B. Sprinson, Biochem, Biophys. Res. Commun. 56, 471 (1976). 14 j. Saltzgaber-Mueller and G. Schatz, J. Biol. Chem. 253, 305 (1978). 1~ E. Keyhani and J. Keyhani, Arch. Biochem. Biophys. 167, 596 (1975). 16 L. Kov~i~, Biochim. Biophys. Acta 346, 101 (1974). 42 BIOGENESIS OF MITOCHONDRIA  synthesis of cytochrome Cl, on the other hand, allow one to define the factors which control the insertion of a single heme protein into the mitochondrial inner membrane.