By Francesco H. Romano, Andrea Russo
Biocatalysis encompasses using enzymes or complete phone structures for effecting the conversion of on hand, low-cost beginning fabrics to excessive price items. Enzymes usually demonstrate beautiful selectivity, rather chemo-, enantio- and regio-selectivity, making them appealing catalysts for a variety of chemical alterations. Enzymes additionally commonly function less than gentle stipulations of pH and temperature resulting in the formation of goods of excessive purity. because of those merits, enzymes and entire cells are discovering wider program in parts equivalent to the construction of intermediates for prescribed drugs, positive chemical compounds, agrichemicals, novel fabrics, diagnostics, biofuels and function chemical compounds. This ebook provides modern examine within the box.
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Additional info for Biocatalysis Research Progress
Typically proteins display an absorbance peak around 280 nm, a linear increase of the adsorbance at this wavelength, plotted against the number of deposited layers, would confirm a proper and uniform deposition. Another useful technique, which does not require expensive and complicated instruments, is QCM. QCM is based on the properties of piezoelectric quartz crystals to vary frequency of their resonant oscillations according to the state of the resonator surface. The deposition/adsorption of molecules onto the surface of the quartz determines a decrease in the oscillating frequency.
They had shown the possibility to realize molecular architecture with this method. Complicated structures with alternation of layers of different molecules were realized. Such systems were used for studying the energy transfer processes. Layers of donors and acceptors were separated by spacer layers of different thickness. Lb Films of Enzymes: Fromherz Trough Application of the LB technique for the deposition of enzyme films meets the same difficulties as for the most of other proteins [Erokhin, 2002].
Developed an approach to protect substances encased in LbL films [Shutava, 2006]. This approach is based on the deposition of a layer which is permeable for the molecules of interest and acts as a barrier for pollutants on the top of the biocatalytic film of interest. Namely, hemoglobin/polyelectrolyte films were adopted as model systems, as hemoglobin has some biocatalytical properties and can be irreversibly destroyed by hydrogen peroxide. It was demonstrated that a catalase layer deposited on the top of the multilayer acts as a protective antioxidant barrier lowering the amount of hydrogen peroxide reaching the 30 Laura Pastorino and Svetlana Erokhina hemoglobin layers in the film depth.